| 石瑾，武云霞.神经生长因子对人牙周膜细胞增殖与分化作用的研究[J].山西医科大学学报,2007,38(11):993~995 |
| 神经生长因子对人牙周膜细胞增殖与分化作用的研究 |
| Effect of nerve growth factor on proliferation and di fferentiation of human periodontal ligament cells in vitro |
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| DOI： |
| 中文关键词: 神经生长因子 人牙周膜细胞 增殖 分化 |
| 英文关键词: nerve growth factor human periodontal ligament ce lls proliferation differentiation |
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| 中文摘要: |
| 目的 研究神经生长因子对体外培养的人牙周膜细胞增殖和分化作用的影响。
方法 采用四唑盐比色法（MTT）和碱性磷酸酶（ALP）活性检测法,测定 不同浓度(0.1 U/ml、1 U/ml、10 U/ml、
100 U/ml)的神经生长因子对体外培养的第5-6代人 牙周膜细胞增殖及碱性磷酸酶活性的作用。结果 与对照组(2%FBS的DME M培养液)相比,1，10，100 U/ml的神经生长因子都可促进人牙周膜细胞的增殖,但10 U/ml作 用最显著；100 U/ml的神经生长因子可显著促进碱性磷酸酶的活性(P<0.01)。结论 不同浓度的神经生长因子可显著促进人牙周膜细胞的增殖与分化。 |
| 英文摘要: |
| Objective To evaluate the effects o f nerve growth factor(NGF) on proliferation and differentiation of human periodo ntal ligament cells(hPDLCs) in vitro. Methods hPDLCs were cultured by explant
separation method. Th e fifth to sixth generation hPDLCs were cultured with 0.1 U/ml,1 U/ml,10 U/ml ,100 U/ml NGF for 24 h in experiment groups. Colorimetric MTT method and enzy me dynamics method were used to observe the proliferation of hPDLCs and alkaline phosphatase (ALP) activities. Results Compared with control group(cultured with 2% FBS DME M), the cell proliferation significantly increased in 3 experiment groups(cult ured with 1, 10, 100 U/ml NGF DMEM), highest in 10 U/ml NGF DMEM group (P< 0.05). ALP activities also significantly increased in 3 experiment groups (cu ltured with 1, 10, 100 U/ml NGF DMEM), highest in 100 U/ml NGF group(P<0.01).Conclusion Different concentrations of NGF c ould promote proliferation and differentiation of hPDLCs in vitro. |
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