文章摘要
张会丰1,2,王卫平1,杨毅1,张艳玲2,皮亚雷 2,田晓瑜2,蒋秀芳2.地塞米松对大鼠生长板软骨细胞TGF-β1表达的影响[J].山西医科大学学报,2007,38(11):996~998
地塞米松对大鼠生长板软骨细胞TGF-β1表达的影响
Effect ofdexamethasone on TGF-β1 expression in the growth plate of rats
  
DOI:
中文关键词: 糖皮质激素  生长板  转化生长因子β  骨骼纵向生长
英文关键词: glucocorticoid  growth plate  transforming growth factor-β  longitudinal g rowth of the bone
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作者单位
张会丰1,2,王卫平1,杨毅1,张艳玲2,皮亚雷 2,田晓瑜2,蒋秀芳2 1复旦大学儿科医院儿童保健科上海200032
2 河北医科大学第二医院儿科 
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中文摘要:
      目的 观察药理剂量地塞米松对大鼠生长板软骨细胞TGF-β1表达的影响,探讨地塞米松抑制大 鼠骨骼生长的机制。 方法 3周龄清洁级纯系雄性SD大鼠,随机分为实验 组12只和对照组8只。实验组大鼠腹腔注射地塞米松(200 μg/100 g,10 d)处理,对照 组注射等容积的生理盐水。胫骨切片做TGF-β1免疫组化分析。 结果 对照组大鼠生长板TGF-β1免疫组织化学染色切片肥大带软骨细胞呈现免疫阳性反应。 地塞米松处理使大鼠生长板肥大软骨细胞TGF-β1表达增加。 结论 药理剂量地塞米松促进肥大带软骨细胞表达TGF-β1,从而抑制骨骼纵向生长。
英文摘要:
      Objective To investigate the effect of pharmacological doses of dexamethasone on expressio n of transforming growth factor-β1 (TGF-β1) in the growth plate of rats, and to explore its mechanism for inhibiting the longitudin al growth of the bone in rats. Methods Twenty 3 week old male SD rats were randoml y divided into two groups: dexamethasone group and control group. The rats were intraperitoneally injected dexamethasone (200 μg /100 g body weight) for 10 din dexamethasone group, and same volume of vehicle in control group. The proximal tibiae were excised. TGF-β1 expression was determined by immunohistochemis try. Results Compared with control group, TGF-β1 expressio n increased significantly in hypertrophic chondrocytes in growth plates of rats in dexamethasone group. Conclusion The pharmacological doses of dexamethasone could inhibit the longitudinal growth of bone by increasing the expression of TGF-β1.
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