文章摘要
刘立新1, 2, 3 *,黄帅1,张海燕1,3,田小霞2.肝纤维化肝硬化患者肝组织中IGFBP 2,6,7的表达及意义[J].山西医科大学学报,2009,40(1):1~5
肝纤维化肝硬化患者肝组织中IGFBP 2,6,7的表达及意义
Expression of IGFBP 2, 6,and 7 in patients with hepatic fibrosis and cirrhosis and its significance
  
DOI:
中文关键词: 肝纤维化  肝硬化  胰岛素样生长因子结合蛋白
英文关键词: hepatic fibrosis  hepatic cirrhosis  insulin like growth factor binding protein
基金项目:国家自然科学基金资助项目(30740031);国家教育部新世纪优秀人才支持计划资助项目(NCET-06-0264)
作者单位
刘立新1, 2, 3 *,黄帅1,张海燕1,3,田小霞2 1山西医科大学第一临床医学院科研实验中心太原0300012山西医科大学肝病研究所3省部共建教育部细胞生理学重点实验室*通讯作者E mail:lixinliu6@hotmail.com 
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中文摘要:
      目的 研究胰岛素样生长因子结合蛋白(IGFBP)在肝纤维化和肝硬化患者肝组织中的表达、分布和意义。 方法 对18例肝纤维化、18例肝硬化患者肝组织和6例对照组(为手术切除的肝血管瘤外的正常肝组织)标本分别进行HE染色、VG染色,以及TGF β1、TGFRΙ、胶原纤维、IGFBP 2, 6, 7及CD14的免疫组织化学染色,并将其通过图像分析系统进行半定量分析和相关分析。 结果 对照组正常肝组织IGFBP 2, 6, 7的表达表现为极少数肝细胞、中央静脉和胆管上皮细胞有阳性染色,在肝纤维化组和肝硬化组的表达主要见于肝窦内和纤维间隔。并且IGFBP 2, 6, 7在对照组正常肝组织、肝纤维化组和肝硬化组肝组织的表达逐渐增高,组间差异有统计学意义(P<0.05)。定量分析肝组织IGFBP 2, 6, 7的表达分别与肝组织胶原纤维、TGF β1、TGFRΙ、CD14的表达呈正相关。 结论 IGFBP 2, 6, 7在人肝纤维化的发生发展中可能起重要作用。
英文摘要:
      Objective To investigate the expression and distribution of IGFBP 2, 6,and 7 in different stages of hepatic fibrosis and eventually cirrhosis in vivo. Methods Forty two liver specimens were collected from 18 cases of hepatic fibrosis(fibrosis group),18 cases of hepatic cirrhosis(cirrhosis group),and 6 cases of normal hepatic tissues after partial hepatectomy of hepatic hemangioma(control group)and observed by HE staining and VG staining.Expression of TGF β1,TGFRΙ,collagen fiber(Cf),IGFBP 2, 6,and 7 and CD14 was detected and semiquantitatively analyzed by immunohistochemistry staining. Results In control group immunohistochemistry revealed that just a small mount of positive staining of IGFBP 2, 6, and 7 was detected upon a few hepatocytes,central veins,and tube wall of bile duct.However,compared with control group,the expression of IGFBP 2, 6,and 7 significantly up regulated in hepatic sinusoid and around fibrous septa in fibrosis and cirrhosis groups(P<0.05).Furthermore,the expression of IGFBP 2, 6,and 7 was higher in cirrhosis group than in fibrosis group.The high expression of IGFBP 2, 6,and 7 in hepatic tissues was correlated positively with TGF β1,TGFRI,CD14,and collagen fiber(Cf). Conclusion IGFBP 2, 6,and 7 may play an important role in the occurrence and development of hepatic fibrosis and cirrhosis.
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