XIAP抑制剂Embelin体外对人胰腺癌细胞株的抑制效应与机制研究

郑玲1*; 吕寒静1; Mouad Edderkaoui2; Stephen J Pandol2

文章摘要

郑玲1*，吕寒静1，Mouad Edderkaoui2，Stephen J Pandol2.XIAP抑制剂Embelin体外对人胰腺癌细胞株的抑制效应与机制研究[J].山西医科大学学报,2009,40(1):20~23

XIAP抑制剂Embelin体外对人胰腺癌细胞株的抑制效应与机制研究

Inhibitive effect of a XIAP inhibitor Embelin on human pancreatic carcinoma cells and its mechanism

DOI：

中文关键词: Embelin 人胰腺癌细胞株凋亡增殖

英文关键词: Embelin human pancreatic carcinoma cell apoptosis proliferation

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作者	单位
郑玲1*，吕寒静1，Mouad Edderkaoui2，Stephen J Pandol2	1同济大学附属同济医院呼吸科，上海200065；2Department of Medicine,Veterans Affairs Greater Los Angeles Healthcare System and University of California,Los Angeles,CA 90073,USA；*通讯作者,E mail:zhengling6568@yahoo.com

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中文摘要:

目的〓探讨XIAP小分子抑制剂Embelin体外对人胰腺癌细胞株MIAPaCa 2的抑制效应与机制。〓方法〓将生长至第4-12代之间的MIAPaca 2细胞分为DMSO对照组与Embelin实验组，在实验组细胞中加入不同浓度的Embelin共孵育72 h，收集细胞并裂解后取上清液用细胞凋亡试剂盒检测组蛋白(凋亡指标)的OD值水平。MIAPaca 2细胞加入不同浓度的Embelin共孵育60 h后再加入3H胸腺嘧啶继续孵育12 h，取细胞裂解液用同位素γ闪烁计数仪检测3H CPM数值以判断细胞增殖程度。对DMSO对照组与Embelin实验组的组蛋白OD值与3H CPM数值分别进行比较分析。〓结果〓 Embelin实验组的MIAPaCa 2细胞株组蛋白OD值显著高于DMSO对照组而3H CPM数值明显低于DMSO对照组（P<0.01），且实验组Embelin的作用浓度与组蛋白OD值呈正相关（r=0.996,P<0.01）,而与3H CPM数值呈负相关（r=-0.993,P<0.01）。〓结论〓 Embelin呈剂量依赖性地诱导人胰腺癌细胞株MIAPaCa 2凋亡并抑制其增殖，有肿瘤抑制效应。

英文摘要:

Objective〓 To explore the inhibitive effect of Embelin,a small molecule XIAP inhibitor,on human pancreatic carcinoma cells MIAPaCa 2 and its mechanism. 〓Methods〓 MIAPaCa 2 cells of generation 4 to generation 12 were divided into Embelin group and DMSO control group.MIAPaCa 2 cells in Embelin group were cultured with different concentrations of Embelin for 72 h,harvested and lysed.And then histone levels were determined with cell death detection ELISAPLUS kit.MIAPaCa 2 cells were cultured with diffe rent concentrations of Embelin for 60 h,and then cultured with 3H thymidine for 12 h.Then 3H CPM values were measured with scintillation counter in cell lysis supernatants. 〓Results〓 OD values of histone in Embelin group were higher than those in DMSO control group(P<0.01),while 3H CPM values in Embelin group were lower than those in DMSO control group(P<0.01).Embelin concentrations in Embelin group were positively related with histone OD values(r=0.996,P<0.01),but negatively related with 3H CPM values(r=-0.993,P<0.01). 〓Conclusion〓 Embelin may have anti tumor effect on human carcinoma cells by inducing the apoptosis of human pancreatic carcinoma cell MIAPaCa 2 and inhibiting dose dependently its proliferation.

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