文章摘要
刘玉芳,李兴*.高糖环境下脂联素对人肾小管上皮细胞增殖及细胞间黏附分子1表达的影响[J].山西医科大学学报,2009,40(1):23~25
高糖环境下脂联素对人肾小管上皮细胞增殖及细胞间黏附分子1表达的影响
Effects of adiponectin on cell proliferation and expression of intercellular adhesion molecule 1 in human renal tubular epithe lial cells cultured with high D glucose
  
DOI:
中文关键词: 脂联素  肾小管上皮细胞  细胞间黏附分子
英文关键词: adiponectin  renal tubular epithelial cell  intercellular adhesion molecule 1
基金项目:
作者单位
刘玉芳,李兴* 山西医科大学第二临床医学院内分泌科太原030001*通讯作者,E mail:Lixinglaoshi@yahoo.com.cn 
摘要点击次数: 675
全文下载次数: 0
中文摘要:
      目的〓 探讨脂联素(ADPN)对体外高糖培养的人肾小管上皮细胞增殖及细胞间黏附分子1(ICAM 1)表达的影响,揭示脂联素对高糖环境中的肾小管上皮细胞可能的保护作用机制。 〓方法〓 ①将人肾小管上皮细胞用5.5 mmol/L葡萄糖(正常对照组)、 30.0 mmol/L葡萄糖(高糖组)、 5.5 mmol/L葡萄糖+24.5 mmol/L甘露醇(甘露醇组)、 30.0 mmol/L葡萄糖+2.5 μg/ml ADPN(脂联素组)分别培养24 h,48 h,96 h后,运用MTT法测定细胞增殖。②将人肾小管上皮细胞用5.5 mmol/L葡萄糖(正常对照组)、 30.0 mmol/L葡萄糖(高糖组)、 30.0 mmol/L葡萄糖+不同浓度ADPN(ADPN终浓度分别为2.5,5.0,7.5,10.0,15.0和20.0 μg/ml,脂联素组)分别培养48 h后,运用酶联免疫吸附法(ELISA)测定细胞上清液中ICAM 1的含量。 〓结果〓 ①高糖组细胞增殖被抑制,加入低浓度脂联素(2.5 μg/ml)后促进了细胞增殖;②ICAM 1在高糖组表达较高,加入中等以上浓度脂联素(ADPN浓度为10.0,15.0,20.0 μg/ml)ICAM 1的表达减少。 〓结论〓 结果提示脂联素可能通过减少ICAM 1的表达而起到保护肾脏的作用。
英文摘要:
      Objective〓 To explore the protective effects of adiponectin(ADPN)on human renal tubular epithelial cells under the condition of high concentration of D glucose. 〓Methods〓 ①The human renal tubular epithelial cells were cultured with 5.5 mmol/L glucose(control group), 30.0 mmol/L glucose(high glucose group), 5.5 mmol/L glucose+24.5 mmol/L mannitol(mannitol group),30.0 mmol/L glucose+2.5 μg/ml adiponectin(ADPN group),respectively.After culture for 24 h,48 h,96 h,the proliferation activity of human renal tubular epithelial cell was observed by MTT.②The human renal tubular epithelial cells were cultured with 5.5 mmol/L glucose(control group),30.0 mmol/L glucose(high glucose group),30.0 mmol/L glucose+different concentrations of adiponectin(2.5,5.0,7.5,10.0,15.0,20.0 μg/ml,ADPN groups),respectively.The expression of intercellular adhesion molecule 1(ICAM 1) was measured by enzyme linked immunosorbent assay(ELISA)after culture for 48 h. 〓Results〓 The proliferation of human renal tubular epithelial cells decreased in high glucose group,but increased in 2.5 μg/ml adiponectin group.The expression of ICAM 1 increased in high glucose group,but decreased in 10.0,15.0,20.0 μg/ml adiponectin groups. 〓Conclusion〓 The results indicate that adiponectin maybe protect the kidney in patients with diabetes mellitus by decreasing the expression of ICAM 1.
查看全文   查看/发表评论  下载PDF阅读器
关闭