文章摘要
孟丽娟,张秀莲*,张伟华,范星火,陈玮,侯素敏,陶洁,刘晨.再生障碍性贫血小鼠Foxp3的表达及意义[J].山西医科大学学报,2009,40(1):35~38
再生障碍性贫血小鼠Foxp3的表达及意义
Expression of Foxp3 in aplastic anemia mice and its significance
  
DOI:
中文关键词: 再生障碍性贫血  免疫介导  Foxp3  RT PCR  免疫组化
英文关键词: aplastic anemia  immune mediated method  Foxp3  RT PCR  immunohistochemistry
基金项目:陕西省自然科学基金国际合作资助项目(2005KW-15)
作者单位
孟丽娟,张秀莲*,张伟华,范星火,陈玮,侯素敏,陶洁,刘晨 山西医科大学第一临床医学院血液科太原030001*通讯作者E mail:zxlsj@126.com 
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中文摘要:
      目的〓 探讨再生障碍性贫血(简称再障)小鼠外周血单个核细胞调节T细胞转录因子Foxp3的表达及其意义。 〓方法〓 ①采用免疫介导(γ射线照射和淋巴细胞输入)的方法建立再障小鼠模型。②RT PCR方法检测再障小鼠外周血单个核细胞Foxp3 mRNA的表达。③免疫组织化学方法检测再障小鼠脾脏组织Foxp3蛋白水平的表达。 〓结果〓 ①再障小鼠外周血单个核细胞Foxp3 mRNA的表达较正常小鼠降低(18.90±2.45 vs 57.27±5.71,P<0.05)。②Foxp3蛋白表达水平在再障小鼠脾脏中较正常小鼠低(19.2±10.0 vs 51.4±38.8,P<0.05)。 〓结论〓 Foxp3在再障小鼠外周血中及脾脏组织中表达明显减少,提示Foxp3的表达降低可能参与再生障碍性贫血的免疫发病机制。
英文摘要:
      Objective〓 To explore the expression of transcription factor Foxp3 of regulatory T cell in peripheral blood mononuclear cells in aplastic anemia mice. 〓Methods〓 ①Immune mediated method(γ ray radiation and lymphocytes injection)was used to establish the model of aplastic anemia in BALB/c mice. ②The expression of Foxp3 mRNA in peripheral blood mononuclear cells in aplastic anemia mice was measured by RT PCR.③The expression of Foxp3 protein in spleen of aplastic anemia mice was detected by immunohistochemical method. 〓Results〓 ①The expression of Foxp3 mRNA in peripheral blood mononuclear cells in aplastic anemia mice was lower than that in normal mice(18.90±2.45 vs 57.27±5.71,P<0.05).②The expression of Foxp3 protein in the spleen of aplastic anemia mice was lower than that in normal mice(19.2±10.0 vs 51.4±38.8,P<0.05). 〓Conclusion〓 The results suggest that the expression of Foxp3 may be involved in the immune pathogenesis of aplastic anemia.
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