文章摘要
袁建林,于磊,张更,王立国,武国军*.AMACR siRNA表达载体对前列腺癌PC 3细胞生物学特性的影响[J].山西医科大学学报,2009,40(1):39~42
AMACR siRNA表达载体对前列腺癌PC 3细胞生物学特性的影响
Effect of AMACR RNAi eukaryotic expression vectors on biological characteristics of PC 3 cell line
  
DOI:
中文关键词: 前列腺肿瘤  AMACR  RNA干扰
英文关键词: prostate neoplasm  α methylacyl CoA racemase  RNA interference
基金项目:陕西省自然科学基金国际合作资助项目(2005KW-15)
作者单位
袁建林,于磊,张更,王立国,武国军* 第四军医大学西京医院泌尿外科西安710032*通讯作者E mail:wuguojun@fmmu.edu.cn 
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中文摘要:
      目的〓 观察转染α-甲酰辅酶A消旋酶(AMACR)干涉载体后对前列腺癌PC 3细胞系生长状态的影响。 〓方法〓 用脂质体将pSilencer/AMACR1转染前列腺癌PC 3细胞,通过细胞计数、MTT、软琼脂克隆形成实验检测细胞的增殖能力,流式细胞仪分析细胞周期,Annexin Ⅴ FITC检测细胞凋亡。 〓结果〓 siRNA干涉载体瞬时转染PC 3前列腺癌细胞系后,36 h细胞增殖率开始明显下降,软琼脂集落形成减少,流式细胞分析示细胞周期阻滞在G0/G1期(88.6%),Annexin Ⅴ FITC法示早期凋亡细胞率68.3%。 〓结论〓 利用RNA干涉技术抑制了前列腺癌PC 3细胞系的增生,可能会成为一种有效的前列腺癌基因治疗手段。
英文摘要:
      Objective〓 To investigate the influence of expression of α methylacyl CoA racemase(AMACR)on growth of PC 3 cells. 〓Methods〓 Using LipofectAmineTM 2000 reagent,RNAi eukaryotic expression vectors pSilencer/AMACR1 and control vector pSilencer Control were transfected into the human prostate cancer cell line PC 3.The proliferative ability of the transfected cells was determined by cell counting,MTT analysis and soft agar assay.Cell cycle was analyzed with flow cytometry.Apoptotic cells were quantified by staining with FITC conjugated Annexin Ⅴ. 〓Results〓 After the PC 3 cells were transfected with pSilencer/AMACR1,their proliferation rate began to decrease significantly after 36 h culture.However,the cells transfected with pSilencer Control and wild type cells showed the same proliferation rate.The average colony forming decreased compared with PC 3 cells(P< 0.05).Cell cycle analysis showed the number of pSilencer/AMACR1 cells in G0/G1 phase of transfected cells was 88.6%.Approximately 68.3% PC 3 cells were induced into apoptosis. 〓Conclusion〓 The growth of PC 3 cell could be inhibited by pSilencer/AMACR1,which provide us an effective gene therapy for the prostate carcinoma.
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