文章摘要
田秀珠*,土增荣,王丽媛,毕星宇,马淑状,石左萍.人成熟卵母细胞冻融的研究[J].山西医科大学学报,2009,40(1):43~46
人成熟卵母细胞冻融的研究
Study on frozen thawed mature oocytes derived from human
  
DOI:
中文关键词: 人卵母细胞  慢速冷冻  蔗糖浓度  胚胎
英文关键词: human oocyte  slow freezing  sucrose concentrations  embryo
基金项目:山西省归国人员科研基金资助项目(200372)
作者单位
田秀珠*,土增荣,王丽媛,毕星宇,马淑状,石左萍 山西医科大学第一临床医学院妇产科,太原030001
*通讯作者E mail:Tianxiuzhu2@Hotmail.com 
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中文摘要:
      目的〓 探讨冷冻保护剂中不同蔗糖浓度对人成熟卵母细胞冻融的影响。 〓方法〓 根据冷冻液中蔗糖浓度的不同,将收集到的人成熟卵母细胞分为0.1 mol/L组、0.2 mol/L组、0.3 mol/L组,分别采用程序化慢冻-快融法,比较三组人成熟卵母细胞复苏后的存活情况及其行单精子卵泡浆内注射(ICSI)后受精率、卵裂率和优质胚胎形成率。 〓结果〓 0.1 mol/L组、0.2 mol/L组、0.3 mol/L组解冻后的存活率分别为23.1%,40.8%,59.4%,三组比较差异有统计学意义(P<0.05);而受精率、卵裂率、优质胚胎形成率三组间比较差异无统计学意义(P>0.05)。 〓结论〓 提高蔗糖浓度能明显提高人成熟卵母细胞解冻的存活率。
英文摘要:
      Objective〓 To explore the effects of different concentrations of sucrose in cryoprotectant solution on the frozen thawed human mature oocytes. 〓Methods〓 The human mature oocytes were divided into 3 groups according into the concentrations of sucrose in cryoprotectant solution:group A(0.1 mol/L),group B(0.2 mol/L)and group C(0.3 mol/L).The survival rate,fertilization rate, cleavage rate and formation percentage of high quality embryo were analyzed by slow freezing and quick thawing method. 〓Results〓 The survival rates were 23.1%,40.8%,59.4% in group A,group B and group C,respectively(P<0.05).But the fertilization rate,cleavage rate and formation percentage of high quality embryo showed no significant difference between 3 groups(P>0.05). 〓Conclusion〓 Rising concentrations of sucrose in the cryoprotectant solution could obviously enhance the survival rate of human mature oocytes.
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