文章摘要
宋晓媛,马平.前列地尔对大鼠肢体缺血再灌注后脊髓神经元细胞凋亡的影响[J].山西医科大学学报,2009,40(2):97~99
前列地尔对大鼠肢体缺血再灌注后脊髓神经元细胞凋亡的影响
Effects of PGE1 on apoptosis of spinal cord neural cell after ischemia reperfusion of hind limbs in rats
  
DOI:
中文关键词: 肢体缺血再灌注  脊髓  凋亡  前列地尔
英文关键词: limb ischemia reperfusion  spinal cord  apoptosis  PGE1
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作者单位
宋晓媛,马平 山西医科大学第二临床医学院麻醉科太原030001 
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中文摘要:
      目的 探讨前列地尔对大鼠肢体缺血再灌注脊髓神经元细胞凋亡的影响。 方法 成年健康SD大鼠72只,随机分为3组:假手术组(sham组,n=24)、缺血再灌注组(IR组,n=24)、前列地尔组(PGE1组,n=24)。3组大鼠均复制实验性大鼠肢体缺血再灌注损伤模型,采用HE染色观察脊髓L1-5节段的病理学改变,TUNEL法检测脊髓细胞凋亡指数(AI),免疫组化检测脊髓神经元Bcl 2、caspase 3表达的变化。 结果 与sham组比较,IR组和PGE1组Bcl 2、caspase 3阳性细胞率、AI都显著增加(P<0.01),Bcl 2在IR组内于再灌注12 h表达达高峰;caspase 3和AI在IR组内于再灌注24 h表达达高峰。IR组和PGE1组caspase 3与AI呈正相关。与IR组比较,PGE1组Bcl 2阳性细胞率增加,caspase 3阳性细胞率和AI减少(P<0.05)。 结论 前列地尔可能通过上调Bcl 2表达和下调caspase 3表达使脊髓神经元凋亡减轻,从而对大鼠肢体缺血再灌注后脊髓神经元有一定的保护作用。
英文摘要:
      Objective〓 To investigate the effects of PGE1 on apoptosis of spinal cord neural cells after ischemia reperfusion of hind limbs in rats. 〓Methods〓 Seventy two SD rats were randomly divided into 3 groups:sham operation group(sham group,n=24),ischemia reperfusion group(IR group,n=24),PGE1 group(n=24).Model of limb ischemia reperfusion was established in all rats.The pathological changes of spinal cord were assessed by HE staining.The apoptosis index(AI)of spinal cord neural cells was measured by TUNEL method.The expression of Bcl 2 and caspase 3 was assessed by immunohistochemical method. 〓Results〓 The expression of Bcl 2,caspase 3 and apoptosis index of spinal cord neural cells were significantly higher in IR group and PGE1 group than in sham group(P<0.01).The expression of Bcl 2 peaked after reperfusion for 12 h.The expression of caspase 3 peaked after reperfusion for 24 h,while apoptotic cells were the most.In IR group and PGE1 group,caspase 3 was positively correlated with apoptosis index.The expression of Bcl 2 was significantly higher in PGE1 group than in IR group,while the expression of caspase 3 and AI was significantly lower(P<0.05). 〓Conclusion〓 PGE1 can relieve the spinal cord neural cell apoptosis by up regulating Bcl 2 expression and down regulating caspase 3 expression,and then protect spinal cord neuron after hind limb ischemia reperfusion injury.
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