文章摘要
孙纪元1,李纪鹏2,缪珊1,王四旺1*,谢艳华1,杨倩1.染料木素对去卵巢大鼠海马神经元的保护作用[J].山西医科大学学报,2009,40(4):291~294
染料木素对去卵巢大鼠海马神经元的保护作用
Protective effect of genistein on hippocampus neurons in ovariectomized rats
  
DOI:
中文关键词: 染料木素  〓雌激素  〓去卵巢  〓海马神经元  〓免疫荧光染色
英文关键词: genistein  〓estrogen  〓ovariectomization  〓hippocampus neuron  〓immunofluorescence staining
基金项目:陕西省中医药管理局科研基金资助项目(2006-082)
作者单位
孙纪元1,李纪鹏2,缪珊1,王四旺1*,谢艳华1,杨倩1 1第四军医大学药学系药物研究所西安710032
2第四军医大学西京医院胃肠外科
*通讯作者Tel:029-84774748E mail:siwang wang@yahoo.com 
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中文摘要:
      目的〓 观察染料木素对去卵巢大鼠海马神经元的影响。 〓方法〓 75只SD雌性大鼠随机分为5组并行去卵巢术,分别为对照组(行假手术)、模型组(OVX)、尼尔雌醇组(E2,1.5 mg/kg)和染料木素治疗组(GenH组,14 mg/kg;GenL组,7 mg/kg)。以上给药除尼尔雌醇每周1次外,其他各组给药均为每日1次。给药4周后对海马区nNOS神经元数量、nNOS基因和蛋白质的水平进行测定。 〓结果〓 去卵巢4周后,OVX组大鼠大脑海马区nNOS神经元数量、nNOS基因和蛋白质的水平较对照组明显降低,不同剂量的染料木素和尼尔雌醇替代治疗均能显著增加上述指标水平(P<0.01),且不同浓度染料木素和尼尔雌醇在增加上述指标水平上没有统计学差异。 〓结论〓 染料木素对去卵巢大鼠海马神经元具有一定的保护作用。
英文摘要:
      Objective〓 To investigate the effects of genistein on hippocampus neurons in ovariectomized rats. 〓Methods〓 Seventy five Sprague Dawley female rats were randomized into five groups as follows:sham group,ovariectomized(OVX)group,nilestriol(E2)group,14 mg/kg genistein group and 7 mg/kg genistein group.The OVX rats were subcutaneously injected with nilestriol at a dosage of 1.5 mg/kg once a week in E2 group.The OVX rats were given 14.0 mg/kg and 7.0 mg/kg genistein once a day in genistein groups,respectively.The level of nNOS,the relative expression of nNOS gene and protein were determined after treatment for 4 weeks. 〓Results〓 Compared with OVX group,the level of nNOS,the relative expression of nNOS gene and protein increased significantly in genistein groups and E2 group(P<0.01),but there was no significant difference between genistein groups and E2 group. 〓Conclusion〓 Genistein could protect the hippocampus neurons in ovariectomized rats.
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