文章摘要
孙玲,阴怀清*,赵旭晶,房清丽,武师润,阴崇娟.不同剂量的rhG CSF对新生大鼠缺氧缺血性脑损伤后脑组织VEGF的影响[J].山西医科大学学报,2009,40(4):299~301
不同剂量的rhG CSF对新生大鼠缺氧缺血性脑损伤后脑组织VEGF的影响
Effects of different doses of rhG CSF on VEGF in brain tissue after hypoxic ischemic brain damage in neonatal rats
  
DOI:
中文关键词: 重组人粒细胞集落刺激因子  〓缺氧缺血性脑损伤  〓血管内皮生长因子
英文关键词: recombinant human granulocyte colony stimulating factor  〓hypoxic ischemic brain damage  〓vascular endothelial growth factor
基金项目:太原市科技局科技兴市基金资助项目(0705026)
作者单位
孙玲,阴怀清*,赵旭晶,房清丽,武师润,阴崇娟 山西医科大学第一临床医学院儿科太原030001*通讯作者,E mail:yhq0351@163.com 
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中文摘要:
      目的〓 探讨不同剂量的rhG CSF对新生大鼠缺氧缺血性脑损伤后脑组织VEGF的影响。 〓方法〓 7日龄Wistar大鼠96只随机分为四组:缺氧缺血性脑损伤(HIBD)模型组,rhG CSF小剂量组(60 μg/kg),中剂量组(100 μg/kg),大剂量组(200 μg/kg),每组各24只。每组根据处死时间不同又分为三个亚组:24 h组,48 h组,72 h组,每亚组各8只。各组均建立新生大鼠缺氧缺血性脑损伤模型,治疗组分别在缺氧缺血后即刻给予rhG CSF 60 μg/kg,100 μg/kg,200 μg/kg颈部皮下注射,并连用3 d。各组于不同时间点取脑组织,用免疫组化法检测脑组织中VEGF的表达。 〓结果〓 与HIBD模型组各时间点相比,rhG CSF各剂量组脑组织VEGF表达均明显增加,差异均有统计学意义(P<0.01);同一时间点rhG CSF各剂量组间VEGF表达差异均无统计学意义(P>0.05),但小剂量组各时间点脑组织外观两侧基本对称,而中剂量组及大剂量组缺氧缺血侧脑组织出现水肿,大剂量组较明显。 〓结论〓 rhG CSF治疗可明显增加脑组织VEGF的表达,有利于脑组织结构和功能的恢复,60 μg/kg为较合适的剂量。
英文摘要:
      Objective〓 To explore the effect of different doses of recombinant human granulocyte colony stimulating factor(rhG CSF)on VEGF in brain tissue after hypoxic ischemic brain damage in neonatal rats. 〓Methods〓 A total of 96 seven day old Wistar rats were randomized into four groups(n=24 in each group):hypoxic ischemic brain damage(HIBD)group,low dose rhG CSF group,medium dose rhG CSF group,high dose rhG CSF group.Then each group was divided into three subgroups(n=8 in each subgroup)based on different time points after HIBD(24 h,48 h,72 h).HIBD model was established.The rats were subcutaneously given rhG CSF 60,100,200 μg/kg body weigh for 3 d after HIBD in rhG CSF groups,respectively.Rats were sacrificed at different time points,and then the expression of VEGF in brain tissue was detected with immunohistochemistry. 〓Results〓 Expression of VEGF in brain tissue at different time points in rhG CSF groups increased significantly compared with HIBD group(P<0.01),but there was no statistical difference between rhG CSF groups at the same time point(P>0.05).The hemisphere of bilateral brain was symmetrical in appearance in low dose group,while edema occurred in HIBD side brain in medium and high dose groups. 〓Conclusion〓 The rhG CSF could increase the expression of VEGF in brain tissue after HIBD,which contributes to the structural and functional restoration of brain tissue.The dose of 60 μg/kg body weight is comparatively suitable.
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