文章摘要
钟丽欣,成霄黎.贝伐单抗抑制碱烧伤角膜新生血管的实验研究[J].山西医科大学学报,2009,40(4):326~328
贝伐单抗抑制碱烧伤角膜新生血管的实验研究
Curative effect of subconjunctival injection of bevacizumab on angiogenesis in alkali burn cornea of rats
  
DOI:
中文关键词: 贝伐单抗  〓角膜  〓新生血管
英文关键词: bevacizumab  〓cornea  〓neovascularization
基金项目:
作者单位
钟丽欣,成霄黎 山西医科大学第一临床医学院眼科太原030001 
摘要点击次数: 534
全文下载次数: 0
中文摘要:
      目的〓 评价结膜下注射贝伐单抗(bevacizumab)对大鼠碱烧伤角膜新生血管化的影响。 〓方法〓 将22只成年雄性Wistar大鼠,碱烧伤诱导单眼角膜新生血管模型后随机分为两组:实验组和对照组。实验组(10眼)给予贝伐单抗原液(25 mg/ml)0.02 ml结膜下注射,对照组(10眼)给予结膜下注射等量生理盐水,两组给药时间均为碱烧伤第1天、第3天及第5天,每日1次,共3次。分别采用宏观测量新生血管长度、生长速度及新生血管化面积,显微镜下微血管计数方法研究角膜新生血管形成及抑制情况。 〓结果〓 实验组新生血管生长速度较对照组慢[(1.05±0.62)mm/d vs(2.87±0.34)mm/d,P<0.05];新生血管化面积实验组较对照组小[(30.5±18.8)% vs (57.5±11)%,P<0.05];微血管计数实验组较对照组少,差异均具有统计学意义(1.86±1.54 vs 8.51±2.33,P<0.05)。 〓结论〓 贝伐单抗可抑制大鼠碱烧伤角膜新生血管的增殖。
英文摘要:
      Objective〓 To evaluate the effects of the subconjunctival injection of bevacizumab on angiogenesis in alkali burn cornea of rats. 〓Methods〓 The corneal neovascularization(CNV)model was induced by alkali burns in corneas of 22 Wistar male rats.And then they were divided in two groups:control group and experimental group.The corneas in control group received subconjunctivally 0.02 ml of 0.9% saline solution,while the corneas in experimental group received subconjunctivally 0.02 ml of bevacizumab(25 mg/ml)at day 1,3 and 5 after lesion.The angiogenesis and inhibition of corneal neovascularization(CNV)were detected by calculating microvessl numbers under microscopy,and determining the length,the growth rate and the area of CNV under slit lamp. 〓Results〓 The growth rate of CNV was slower in experimental group than in control group[(1.05±0.62)mm/d vs(2.87±0.34)mm/d,P<0.05].The area of neovascularization of the corneal surface in experimental group was smaller than in control group[(30.5±18.8)% vs (57.5±11)%,P<0.05].The microvessl number was lower in experimental group(1.86±1.54)than in control group(8.51±2.33,P<0.05). 〓Conclusion〓 Bevacizumab may inhibit corneal angiogenesis.
查看全文   查看/发表评论  下载PDF阅读器
关闭