| 钟丽欣,成霄黎.贝伐单抗抑制碱烧伤角膜新生血管的实验研究[J].山西医科大学学报,2009,40(4):326~328 |
| 贝伐单抗抑制碱烧伤角膜新生血管的实验研究 |
| Curative effect of subconjunctival injection of bevacizumab on angiogenesis in alkali burn cornea of rats |
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| DOI： |
| 中文关键词: 贝伐单抗 〓角膜 〓新生血管 |
| 英文关键词: bevacizumab 〓cornea 〓neovascularization |
| 基金项目: |
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| 中文摘要: |
| 目的〓
评价结膜下注射贝伐单抗（bevacizumab）对大鼠碱烧伤角膜新生血管化的影响。
〓方法〓
将22只成年雄性Wistar大鼠，碱烧伤诱导单眼角膜新生血管模型后随机分为两组:实验组和对照组。实验组（10眼）给予贝伐单抗原液（25 mg/ml）0.02 ml结膜下注射，对照组（10眼）给予结膜下注射等量生理盐水,两组给药时间均为碱烧伤第1天、第3天及第5天，每日1次，共3次。分别采用宏观测量新生血管长度、生长速度及新生血管化面积,显微镜下微血管计数方法研究角膜新生血管形成及抑制情况。
〓结果〓
实验组新生血管生长速度较对照组慢[（1.05±0.62）mm/d vs（2.87±0.34)mm/d，P＜0.05]；新生血管化面积实验组较对照组小[（30.5±18.8）% vs （57.5±11）%,P＜0.05]；微血管计数实验组较对照组少,差异均具有统计学意义（1.86±1.54 vs 8.51±2.33,P<0.05）。
〓结论〓
贝伐单抗可抑制大鼠碱烧伤角膜新生血管的增殖。 |
| 英文摘要: |
| Objective〓
To evaluate the effects of the subconjunctival injection of bevacizumab on angiogenesis in alkali burn cornea of rats.
〓Methods〓
The corneal neovascularization(CNV)model was induced by alkali burns in corneas of 22 Wistar male rats.And then they were divided in two groups:control group and experimental group.The corneas in control group received subconjunctivally 0.02 ml of 0.9% saline solution,while the corneas in experimental group received subconjunctivally 0.02 ml of bevacizumab(25 mg/ml)at day 1,3 and 5 after lesion.The angiogenesis and inhibition of corneal neovascularization(CNV)were detected by calculating microvessl numbers under microscopy,and determining the length,the growth rate and the area of CNV under slit lamp.
〓Results〓
The growth rate of CNV was slower in experimental group than in control group[(1.05±0.62)mm/d vs(2.87±0.34)mm/d,P＜0.05].The area of neovascularization of the corneal surface in experimental group was smaller than in control group[(30.5±18.8)% vs (57.5±11)%,P<0.05].The microvessl number was lower in experimental group(1.86±1.54)than in control group(8.51±2.33,P<0.05).
〓Conclusion〓
Bevacizumab may inhibit corneal angiogenesis. |
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