| 张志勇,席啸虎,康曌,高建平*.日本毛连菜中多糖的含量测定[J].山西医科大学学报,2009,40(4):329~331 |
| 日本毛连菜中多糖的含量测定 |
| Determination of polysaccharide in Picris japoncia Thunb |
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| DOI： |
| 中文关键词: 日本毛连菜 〓多糖 〓含量测定 |
| 英文关键词: Picris japoncia Thunb 〓polysaccharide 〓determination |
| 基金项目:山西医科大学学生创新基金资助项目(2006045) |
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| 中文摘要: |
| 目的〓
建立药用植物日本毛连菜中多糖的含量测定方法。
〓方法〓
采用水提醇沉法从日本毛连菜中提取得到毛连菜粗多糖，脱色，用苯酚-硫酸法测定日本毛连菜中多糖含量。
〓结果〓
在3.03-15.10 μg/ml范围内具有良好的线性关系,本法平均回收率为(97.55±2.26)%，RSD为2.3%。测定日本毛连菜提取物中总多糖含量以葡萄糖计平均为(5.〗80±0.06)%。
〓〖HTK〗结论〓〖HTSS〗
该方法简便、准确、可靠，可以用于日本毛连菜中多糖的含量测定。 |
| 英文摘要: |
| Objective〓
To establish a method for determining the content of polysaccharide in Picris japoncia Thunb.
〓Methods〓
Polysaccharide was extracted with water,purified with alcohol precipitation and decolored with H2O2.Then the content of polysaccharide was determined by phenols sulfuric acid method.
〓Results〓
The linear range for polysaccharide was 3.03-15.10 μg/ml.The average recovery was(97.55±2.26)%.RSD was 2.3%(n=6).The content of polysaccharide in Picris japoncia Thunb was(5.80±0.06)%(glucose).
〓Conclusion〓
The proposed method is convenient,economic, accurate and reproducible,and can be used to determine the polysaccharide in Picris japoncia Thunb. |
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