文章摘要
陈海云,商中华,钱惠岗,张蓉婧.rhEPO预处理对大鼠肝脏缺血再灌注损伤的作用[J].山西医科大学学报,2007,38(11):981~984
rhEPO预处理对大鼠肝脏缺血再灌注损伤的作用
Effect of rhEPO preconditioning on hepatic ischemia reperfusion injury in rats
  
DOI:
中文关键词: 再灌注损伤  人重组促红细胞生成素  预处理  肝脏
英文关键词: reperfusion injury  recombinant human erythropoietin  preconditioning  liver
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作者单位
陈海云,商中华,钱惠岗,张蓉婧 山西医科大学 第二临床医学院普外科太原 030001 
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中文摘要:
      目的 探讨人重组促红细胞生成素(rhEPO)预处理对大鼠肝脏缺血再灌注损伤中的保护作用及其 预处理较为合适的剂量。 方法 Wistar大鼠36只,随机分为对照组A、实 验组B和C,每组12只。A组只给予夹闭肝蒂,B组和C组分别于缺血前30 min皮下注射r hEPO 5 000 U/kg和1 000 U/ kg, 在缺血45 min及再灌注2 h,取血清及肝组织行AST、ALT 、TNF α、IL 1β检测及HE染色、NF κB的活化程度及计算肝细胞凋亡指数(AI)。 结果 与A 组比较,B、C组肝细胞索排列较好,细胞坏死程度不明显。AST 、ALT和TNF α、IL 1β及NF κB活化程度、AI在A组和B、C组之间均有显著差异(P <0.05),而在B、C组之间无统计学差异(P>0.05)。 结论 rhEPO对缺血再灌注损伤的肝脏具有保护作用,且以低剂量(1 000 U/kg)较为合适。
英文摘要:
      Objective To explore the protective effect of rhEPO on hepatic ischemia reperfusion injury in rats and its suitable dosage. Methods Thirty six Wistar rats were randomly divided into th ree groups: control group (group A,n=12), rhEPO preconditioning groups (gro ups B and C, n=12 in each group).Liver pedicles were occlused in group A. I n groups B and C, the rats were subcutaneou sly injected rhEPO 5 000 U/kg and 1 000U/kg at 30 min before ischemia, respecti vely. After 45 min ischemia and 2 h reperfusion,the rats were killed. Serum as partic acid aminotransferase(AST) and alanine aminotransferase (ALT), tumor necr osis factor α(TNF α) and interleukin 1β(IL 1β) were measured, and liver hi stopathology was observed by HE staining.Nuclear factor κB(NF κB) was detect ed by immunohistochemistry, and apoptotic index was calculated by TUNEL. Results The parenchy mal hepatic cell necrosis and structure derangement of hepatic lobules in groups B and C were lighter than in group A. Compared with group A, serum AST, ALT, TN F α,IL 1β significantly decreased (P<0.05),but there was no significant difference between groups B and C (P>0.05).NF κB was significantly higher in groups B and C than in group A(P<0.01), and AI was significantly lower in groups B and C than in group A(P<0.05). Conclusion RhEPO could protect the liver from ischemia reperfusion injury in rats,and 1 000 U/kg is a suitable dose.
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