文章摘要
杨喜花1,陈敏2.沙棘叶中总黄酮的提取和纯化[J].山西医科大学学报,2007,38(11):1005~1009
沙棘叶中总黄酮的提取和纯化
Extraction and purification of total flavones from leaves of Hippophae rhamnoi des L
  
DOI:
中文关键词: 提取  纯化  大孔吸附树脂  沙棘叶  总黄酮
英文关键词: extraction  purification  macroporous resin  leaves of Hippophae rhamnoides L  total flavones
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作者单位
杨喜花1,陈敏2 1山西医科大学公共卫生学院营 养与食品卫生学教研室 太原0300012中国农业大学食品科学与营养工程学院 
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中文摘要:
      目的 研究不同方法对沙棘叶总黄酮的提取效果以及大孔吸附树脂对沙棘叶总黄酮的纯化性能及纯 化条件。 方法 采用索氏提取法、超声提取法和微波提取法提取沙棘叶 总黄酮,用X 5大孔吸附树脂对沙棘叶总黄酮进行纯化,并用紫外分光光度法测定其含量。 结果 索氏提取法提取6 h的提取率为19.78%,超声提取10 min的提取 率 为19.19%,微波提取10 min的提取率为24.19%;X 5大孔吸附树脂对沙棘叶总黄酮的纯化 性能较佳,其对沙棘叶总黄酮的纯化条件为:上样液浓度1.159-1.545 mg/ml,上样 液pH值4 .06,洗脱剂为50%乙醇。 结论 循环超声提取10 min即与索氏提取6 h的 提取效果相当,而微波提取技术大大缩短了提取时间,而且提取率有所增加;X 5树脂适合 应用于沙棘叶总黄酮的纯化。
英文摘要:
      Objective To study the extraction effect of total flavones from leaves of Hippophae rhamnoides L. by different methods and the adsorption function of macroporous resin for the purification of total flavones in leaves of Hipp ophae rhamnoides L. Methods The Soxhlet, circulative ultrasonic wave, and microwave were us ed for flavones extraction, X 5 resin was used to purify the total flavones, an d UV spectrophotometry was used to analyze the contents of total flavones. Results The extraction rates were 19.78% with Soxhlet for 6 h, 19.19%with circu lative ultrasonic wave for 10 min and 24.19% with microwave for 10 min. X 5 re sin had capability of selective adsorption of total flavones from the leaves of Hippophae rhamnoides L. The optimal adsorption were 1.159-1.545 mg/ml of solution concentration at pH 2, and the optimal desorption was 50% e thanol as eluting solvent. Conclusion The effect of circulative ultrasonic extraction for 10 min is almost equal to Soxhlet extraction for 6 h. And the effect of microwave extraction is even faster and more efficient. X 5 resin has favorable effect of adsorption an d desorption for purifying total flavones from leaves of Hippophae rhamnoides L.
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