文章摘要
张俊芳1,侯磊2,郭芬1,祁金顺1*.大鼠在体海马CA1区长持续长时程增强的记录[J].山西医科大学学报,2009,40(1):6~9
大鼠在体海马CA1区长持续长时程增强的记录
Recordings of late phase long term potentiation in rat hippocampal CA1 area in vivo
  
DOI:
中文关键词: 长持续长时程增强  海马  在体记录  场兴奋性突触后电位
英文关键词: late phase long term potentiation  hippocampus  in vivo recording  field excitatory postsynaptic potential
基金项目:国家自然科学基金资助项目(30740095);山西省自然科学基金资助项目 (200601105); 山西省青年科学基金资助项目(2008021045-2)
作者单位
张俊芳1,侯磊2,郭芬1,祁金顺1* 1山西医科大学生理教研室太原0300012山西省肿瘤医院麻醉科*通讯作者Tel:0351-4135091E mail:jinshunqi2006@yahoo.com 
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中文摘要:
      目的 记录双电极绑定条件下多组高频刺激(HFSs)诱导的大鼠在体海马CA1区长持续长时程增强(L LTP)现象。 方法 雄性Wistar大鼠乌拉坦麻醉后固定于脑立体定位仪上。埋置脑室导管并安装绑定的刺激/记录电极。引导基础性场兴奋性突触后电位(fEPSP)和施加多组HFSs诱导L LTP。 结果 绑定后的刺激和记录电极能稳定地引起海马CA1区fEPSP。基础性fEPSP记录可保持长时间稳定。有效的多组HFSs可有效诱导至少维持3 h以上的L LTP,成功率高达65%。脑室注射β淀粉样蛋白(Aβ)对L LTP的维持显示出明显的压抑作用。 结论 采用绑定的刺激/记录电极进行L LTP记录简便易行。利用多组HFSs方案诱导L LTP成功率较高。
英文摘要:
      Objective〓 To record the late phase long term potentiation(L LTP)in the CA1 area of rats in vivo by using electrodes binding technique and delivering multiple high frequency stimulations(HFSs). 〓Methods〓 Wistar rats were anesthetized with urethane and fixed with the stereotaxic device for acute surgery.Cannula was implanted into lateral cerebral ventricle,and bound stimulating/recording electrodes were inserted into hippocampal CA1 area. Basal field excitatory postsynaptic potential(fEPSP)and multiple HFSs induced L LTP were recorded. 〓Results〓 fEPSPs were steadily induced by using the electrodes binding technique.Basal fEPSP recording was very stable,lasting for a long period enough to finish the whole experiment.The multiple HFSs successfully induced L LTP,with a higher incidence up to 65% and a longer maintenance for at least three hours.Intracerebroventricular(i.c.v.)injection of amyloid beta(Aβ)suppressed the maintenance of HFSs induced L LTP significantly. 〓Conclusion〓 The electrodes binding technique for recording hippocampal L LTP in vivo is quite simple and convenient. The rates of fEPSP appearance and L LTP induction are kept high by delivering multiple HFSs.
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