文章摘要
于海霞1,邢桦云2,韩德五2,刘霞3,宋励4.肠源性内毒素血症与肝星形细胞在肝纤维化中的作用[J].山西医科大学学报,2009,40(1):26~28
肠源性内毒素血症与肝星形细胞在肝纤维化中的作用
Role of intestinal endotoxemia and hepatic stellate cell in hepatic fibrosis
  
DOI:
中文关键词: 肝纤维化  内毒素  肝星形细胞
英文关键词: hepatic fibrosis  endotoxine  hepatic stellate cell
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作者单位
于海霞1,邢桦云2,韩德五2,刘霞3,宋励4 1山西医科大学医学实验中心太原0300012山西医科大学病理生理学教研室3山西医科大学第二临床医学院老年医学科4山西医科大学组织学与胚胎学教研室 
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中文摘要:
      目的〓 研究IETM与α SMA在肝纤维化中的作用,以进一步了解二者的关系。 〓方法〓 21只Wistar大鼠随机分为对照组(n=6)和肝纤维化组(n=15)。正常对照组饲以正常饮食,肝纤维化组采用复合因子方法饲养大鼠建立肝纤维化模型。饲养4周后,透射电镜下观察肝星形细胞的活化,免疫组化方法观察α 平滑肌肌动蛋白(α SMA)的表达,并测定血浆中的内毒素含量,同时也测定了丙氨酸转氨酶(ALT)、透明质酸(HA)、丙二醛(MDA)的水平。 〓结果〓 4周末透射电镜下肝纤维化组观察到了HSC的活化,免疫组化法观察到了肝纤维化组α SMA表达明显高于对照组,血浆中ET、ALT、HA、MDA的含量肝纤维化组明显高于对照组。 〓结论〓 肝纤维化发生时有肠源性内毒素血症的发生和肝星形细胞的活化,二者之间可能存在一定关联。
英文摘要:
      Objective〓 To investigate the role of intestinal endotoxemia(IETM)and hepatic stellate cells(HSC)in hepatic fibrosis,and to further explore the relationship of IETM and HSC. 〓Methods〓 All rats were divided into two groups:normal control group(n=6)and hepatic fibrosis group(n=15).The rats were fed with normal diet in control group and complex diet(including CCl4,alcohol,etc)in hepatic fibrosis group.After 4 weeks, the rats were executed.The activation of HSC was observed with transmission electron microscope(TEM),and the expression of α SMA in hepatic tissues was examined by immunohistochemical techniques.The changes of plasma endotoxine,ALT,HA,MDA were detected. 〓Results〓 The activation of HSC was observed at the end of 4th week with transmission electron microscope.Immunohistochemical results showed that α SMA expression in hepatic fibrosis group was higher than in normal control group.Plasma ET,ALT,HA,MDA levels in hepatic fibrosis group were significantly higher than in normal control group. 〓Conclusion〓 IETM and the activation of HSC may occur in hepatic fibrosis.
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