文章摘要
刘霄虹,张伟华*,张秀莲.免疫介导的再生障碍性贫血小鼠模型脾脏T bet的表达水平及其意义[J].山西医科大学学报,2009,40(1):32~34
免疫介导的再生障碍性贫血小鼠模型脾脏T bet的表达水平及其意义
Expression of T bet in spleen of immune mediated aplastic anemia mice
  
DOI:
中文关键词: 再生障碍性贫血  动物模型  Th1淋巴细胞  T bet;小鼠
英文关键词: aplastic anemia  animal model  Th1 lymphocyte  transcription factor T bet  mice
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作者单位
刘霄虹,张伟华*,张秀莲 山西医科大学第一临床医学院血液科太原030001*通讯作者E mail:zwhlzh@126.com 
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中文摘要:
      目的〓 探讨在再生障碍性贫血(aplastic anemia,AA)小鼠模型中,T bet蛋白在脾脏的表达水平及意义。 〓方法〓 将40只BALB/c小鼠随机分为正常对照组和AA模型组,每组20只,用免疫组织化学方法检测AA模型组脾脏组织石蜡切片中T bet蛋白表达水平,并对T bet蛋白与骨髓造血功能进行相关性分析。 〓结果〓 ①AA模型组小鼠脾脏组织石蜡切片中T bet蛋白表达水平[(4.25±0.50)%]明显高于正常对照组[(0.78±0.15)%,P<0.01]。②AA模型组小鼠脾脏T bet蛋白表达与中性粒细胞绝对值(ANC)及网织红细胞相对值呈负相关(r分别为-0.510和-0.648,均P<0.05),而与血小板数无明显的线性相关关系。 〓结论〓 在AA发病机制中T bet可能起一定作用。
英文摘要:
      Objective〓 To explore the expression of T bet in spleen of immune mediated aplastic anemia(AA)mice and its significance. 〓Methods〓 Forty BALB/c mice were randomized into 2 groups:normal control group and AA model group.Twenty mice were immune mediated to establish AA mouse models. Expression of T bet protein in spleen of AA bone marrow was measured by immunohistochemistry. 〓Results〓 Expression of T bet protein in spleen was higher in AA mice than in normal controls[(4.25±0.50)% vs (0.78±0.15)%,P<0.01].T bet expression in spleen was negatively correlated with reticulocyte and absolute neutrophil counts. 〓Conclusion〓 The result suggests that T bet maybe participate the hematopoietic failure of AA.
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